Inspect the plate carrier for any liquid spills or debris. Clean immediately with 70% ethanol and a lint-free wipe.
Define your plate layout by selecting the correct plate manufacturer and well format (e.g., Greiner 96 Flat Bottom).
: For those integrating the Safire 2 into a robotic workstation, the Freedom EVO Service Manual contains specific chapters (e.g., Chapter 1.2) on the installation and integration of Safire 2 readers.
Even though the Safire 2 is a discontinued product (official sales ended around March 2014), many laboratories still rely on it. An original is indispensable for several reasons:
Ideal for molecular interaction studies and high-sensitivity G-protein-coupled receptor (GPCR) assays. tecan safire 2 manual pdf
To help you find the exact information you need, could you specify if you are looking for , software setup (XFluor vs. Magellan), or specific assay protocols ?
Set the photomultiplier tube (PMT) gain. For unknown sample concentrations, use the "Optimal Gain" function, which flashes a designated well to calculate a dynamic range that avoids signal saturation. 3. Executing the Run
Set your target wavelengths. For example, for a standard fluorescein assay, set Excitation to 485 nm and Emission to 535 nm.
For cell-based assays in 96-well plates, the optimal focal height is typically 7-8mm from the bottom. The manual provides a stepwise protocol to run a “Z-focus scan” to find the ideal plane, avoiding out-of-focus cell monolayers. Inspect the plate carrier for any liquid spills or debris
Wait for the software to complete its initialization handshake with the microplate reader. Defining an Assay Method in Magellan Select within the Magellan workspace.
If you can tell me (e.g., communication error, low fluorescence signal, or maintenance query), I can provide more targeted advice. If you'd like, I can also:
Place the instrument on a level, vibration-free laboratory bench. Ensure adequate clearance (at least 10 cm) around the cooling fans to prevent overheating.
Optimizing the gain and integration time to maximize sensitivity without saturation. 4. Maintenance and Troubleshooting : For those integrating the Safire 2 into
Configured for glow, flash, and dual-luciferase reporter assays using high-efficiency photon-counting detectors. Plate Compatibility and Environmental Control
Disclaimer: Always follow the specific safety and operational guidelines provided in the manual that accompanied your specific instrument serial number.
Tecan Safire 2 Manual PDF: Comprehensive Guide to Operation and Maintenance
A few websites have uploaded fragments of Safire 2 related documentation, though the complete user manual remains elusive:
When not in use, keep the plate transport closed to prevent dust from settling on the internal optics.
